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IGFBP1 Antibody

SKU: orb1248369

Description

IGFBP1 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: RIPGSPEIRGDPNCQ
TargetIGFBP1
Molecular WeightApprox 30kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

IGFBP1, insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IBP-1, IGF-binding protein 1, IGFBP-1, OTTHUMP00000159561, OTTHUMP00000207990, OTTHUMP00000214348, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, insulin-like growth factor-binding protein 1, placental protein 12

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IGFBP1 Antibody

orb1248369 (0.1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

IGFBP1 Antibody

orb1248369 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IGFBP1 Antibody (orb1248369)

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Available Sizes

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0.1 mg
$ 880.00
DispatchUsually dispatched within 5-10 working days
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