IGF2BP3 Antibody

• Catalog Number: orb1257695
• Category: Antibodies
• Description: IGF2BP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: IGF2BP3
• UniProt ID: O00425
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (orb1257695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (orb1257695) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100 ug extracts of HepG2 cells using 3 ug IGF2BP3 antibody (orb1257695). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (orb1257695) at a dilution of 1:1000.