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Catalog Number | orb1257695 |
---|---|
Category | Antibodies |
Description | IGF2BP3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 70kDa |
Target | IGF2BP3 |
UniProt ID | O00425 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (orb1257695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (orb1257695) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (orb1257695) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 ug extracts of HepG2 cells using 3 ug IGF2BP3 antibody (orb1257695). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (orb1257695) at a dilution of 1:1000.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |