IGF1R/IGF1 Receptor Antibody

SKU: orb1537912

Description

IGF1R/IGF1 Receptor Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IHC-P, IP, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: JBW902
• Immunogen: GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor.
• Target: IGF1R / IGF1 Receptor
• Purification: Protein A purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR, IGF-I receptor, Soluble IGF1R variant 1, Soluble IGF1R variant 2, Type 1 IGF receptor, IGFIR, JTK13

Quick Database Links

Gene Symbol

IGF1R / IGF1 Receptor