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Catalog Number | orb1929029 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB15292 |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 154793 Da |
Target | This IGF1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human IGF1R. |
UniProt ID | P08069 |
NCBI | NP_000866.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Insulin-like growth factor 1 receptor, Insulin-lik Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35 ug/lane).IGF1R (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IGF1R Antibody (N-term K66) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (PBS alone) (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry. Clinical relevance has not been evaluated.