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Catalog Number | orb1939543 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB33711 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
Target | This IGF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the C-terminal region of human IGF1. |
UniProt ID | P05019 |
NCBI | NP_001104755.1, NP_000609.1, NP_001104754.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | IGF1; IBP1; Insulin-like growth factor I; Mechano Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IGF1 Antibody (C-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the IGF1 antibody detected the IGF1 protein (arrow).
Confocal immunofluorescent analysis of IGF1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IGF1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |