IFNA1 Antibody (C-term)

SKU: orb1933446

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21762
• Immunogen: This IFNA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-189 amino acids from the C-terminal region of human IFNA1. Antigen Region: 164-189 aa.
• Target: IFNA1
• Molecular Weight: 21725 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

IFNA1 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the IFNA1 antibody detected the IFNA1 protein (arrow).

Anti-IFNA1 Antibody (C-term) at 1:2000 dilution + Recombinant protein. Lysates/proteins at 20ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

IFNA1

UniProt

P01562

RefSeq (Protein):NP_076918.1, NP_008831.3