IFN-gamma, Human, mAb F12 Antibody

SKU: orb2653060

Description

Monoclonal antibody binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. The antibody does not react with rodent interferons. Possible applications include the purification of human gamma Interferon and in vitro neutralisation. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.

Images & Validation

• Tested Applications: Functional Studies, IA, IHC-Fr, IP
• Dilution Range: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530).
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized product in PBS, containing 300 µg antibody. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
• Buffer/Preservatives: Lyophilized product in PBS, containing 100 µg antibody. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only