IFNB1 Antibody (Biotin)

SKU: orb1272677

Description

IFNB1 Antibody (Biotin)

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hIFN-b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IFN-b as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-b. Western Blot:To detect hIFN-b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIFN-b (human Interferon-beta). Human IFN-β specific antibody was purified by affinity chromatography and then biotinylated.
• Target: IFNB1
• Conjugation: Biotin

Storage & Handling

• Storage: IFN-b antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta

To detect Human IFN-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IFN-beta (orb1272621) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IFN-beta.

Quick Database Links

Gene Symbol

IFNB1

UniProt

P01574

RefSeq:P01574