IFN-alpha, Mouse, mAb F18 Antibody

SKU: orb1109202

Description

Mouse monoclonal antibody to IFN-alpha

Research Area

Infectious Disease & Virology; Immunology & Inflammation

Images & Validation

• Tested Applications: FC, Functional Studies, IA, WB
• Dilution Range: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. For neutralisation of biological activity dilutions have to be made according to the amount of Interferon to be inactivated. The cross reactivity with Human IFN, Mouse IFN-gamma and Mouse IFN-beta was negative.
• Reactivity: Mouse
• Application Notes:
  FC: For intracellular staining of IFN-α, cells can be fixed in 1 % formaldehyde; blocked and permeabilized in 0.2 % saponin, 5 % normal rabbit serum for 30 minutes on ice. (Ref.2) FS: Neutralization of IFN-α by adding 1 ug antibody F18 per mouse i.v. before treatment with 35 ug LPS i.p., decreased the LPS-induced IL-1β serum response. (Ref.3). IA: It has been used as a capture antibody to determine specificity.

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Key Properties

• Clonality: Monoclonal
• Isotype: Rat IgG1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Buffer/Preservatives: Quantities >500 µg will be sent without preservatives and are carrier-free.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IFN-α