IFIT3 Antibody

• Catalog Number: orb1257793
• Category: Antibodies
• Description: IFIT3 Antibody
• Target: IFIT3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
• UniProt ID: O14879
• MW: Observed: 60kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:1000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFIT3 Antibody : P60, IRG2, IFI60, IFIT4, ISG60, R
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IFIT3 antibody (orb1257793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody (orb1257793) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody (orb1257793) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using IFIT3 antibody (orb1257793) at dilution of 1:100. Blue: DAPI for nuclear staining.