IFIT3 Antibody

SKU: orb1257793

Description

IFIT3 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:1000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
• Target: IFIT3
• Molecular Weight: Observed: 60kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IFIT3 Antibody : P60, IRG2, IFI60, IFIT4, ISG60, RIG-G, CIG-49, GARG-49, Interferon-induced protein with tetratricopeptide repeats 3, CIG49, IFIT-3

Western blot analysis of extracts of various cell lines, using IFIT3 antibody (orb1257793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody (orb1257793) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody (orb1257793) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using IFIT3 antibody (orb1257793) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

IFIT3

UniProt

O14879