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Item 1 of 7
IFI16 (CT) Antibody
Catalog Number: orb1672345
| Catalog Number | orb1672345 |
|---|---|
| Category | Antibodies |
| Description | IFI16 (CT) Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC, WB |
| Predicted Reactivity | Primate |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-IFI16 antibody (orb1672345) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Predicted: 88kD Observed: 88 kD |
| Target | IFI16 |
| UniProt ID | Q16666 |
| NCBI | Q16666.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | IFI16 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | IFI16 Antibody: Gamma-interferon-inducible protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
WB Validation in Human and Rat Cell Lines. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Testis. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with orb1672345 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of IFI16 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IFI16 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
