IFI16 Antibody

SKU: orb1672345

Description

IFI16 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-IFI16 antibody (orb1672345) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16.
• Target: IFI16
• Molecular Weight: Predicted: 88kD Observed: 88 kD
• Purification: IFI16 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

IFI16 Antibody: Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator, IFNGIP1

WB Validation in Human and Rat Cell Lines. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Testis. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with orb1672345 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of IFI16 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IFI16 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

IFI16

UniProt

Q16666

RefSeq:Q16666.3