IDO1/IDO Antibody

• Catalog Number: orb1537621
• Category: Antibodies
• Description: IDO1/IDO Antibody
• Target: IDO1 / IDO
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence CVLANWKKKDPNK, from the internal region of the protein sequence according to NP_002155.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1.5 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B1746 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1746 was determined to be 2.5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IDO1, IDO, Indole 2,3-dioxygenase, Indolamine 2,3
• Note: For research use only

IDO1 / IDO antibody (0.5 µg/ml) staining of Human Lymph Node (A), Spleen (B) and Placenta (C) lysates (35 µg protein in RIPA buffer). Detected by chemiluminescence.