Ido1 Antibody

SKU: orb345211

Description

IDO antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, Multiplex Assay, WB
• Dilution Range: ELISA: 1:5000-1:50000, FC: 0.5-1x10^6 cells, IF: 1:50-1:100, IP: 10-100 µL, WB: 1:500-1:1500
• Reactivity: Mouse
• Application Notes:
  Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 2E2.6
• Immunogen: IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.
• Target: Ido1
• Purity: Anti-IDO1 antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. IDO1 antibody is specific for mouse IDO1 protein. Mouse IDO1 does not react with human tissues. Cross-reactivity with IDO1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.95 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido 1, Ido-1, IDO1 antibody, mouse anti-IDO-1 antibody

Quick Database Links

Gene Symbol

Ido1

UniProt

P28776

RefSeq (Protein):NP_032350.1