IDH1 Antibody

SKU: orb1249271

Description

IDH1 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Yeast
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration: 0.3-1µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EPGSRHVGLDIKGQN
• Target: IDH1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IDH1, Idh1p, YNL037C, Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle

orb1249271 (0.5 ug/ml) staining of S. cerevisiae S288c lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein.

Quick Database Links

Gene Symbol

IDH1

UniProt

P28834

RefSeq (Protein):NP_014361.1