IDH1 Antibody (Center)

• Catalog Number: orb1929244
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18238
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:100, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 46659 Da
• Target: This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
• UniProt ID: O75874
• NCBI: NP_005887.2, NP_001269316.1, NP_001269315.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Isocitrate dehydrogenase [NADP] cytoplasmic, IDH,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). IDH1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-IDH1 Antibody (Center) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 47kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate (from left to right), using IDH1 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.