ID-8

SKU: orb1305495

Description

ID-8 is a small molecule inhibitor of DYRK kinases that enables long-term maintenance of embryonic stem cell self-renewal in culture. It is a valuable research tool for studying stem cell biology, pluripotency, and regenerative medicine in vitro.

Research Area

Cell Biology, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 147591-46-6
• MW: 298.29
• Purity: >98%
• Formula: C₁₆H₁₄N₂O₄
• SMILES: COC1=CC=C(C=C1)N1C(C)=C(C2=C1C=C(O)C=C2)[N+]([O-])=O
• Target: Kinase
• Solubility: Soluble in DMSO (up to 10 mg/ml).

Bioactivity

• In Vivo:
  ID-8 maintains the expression of the Nanog gene through the activation of Sox2-Oct3/4, enabling the reversible maintenance of ESC (Embryonic Stem Cells) cultures for prolonged periods. In the presence of Wnt, ID-8 modulates the Wnt/β-catenin signaling pathway, enhancing CBP/β-catenin levels to preserve the undifferentiated state of hESCs (human Embryonic Stem Cells), thereby supporting cell proliferation and survival.
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• Cell Research:
  Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, Dual specificity tyrosine phosphorylation regulated kinase, Dual specificity tyrosine regulated kinase, dual-specificity, DYRK, inhibit, Inhibitor, kinase, ID8, ID-8, ID 8, embryonic, ESC, tyrosine, stem, regulated, phosphorylation

Compound Identifiers

PubChem CID

791637