ICAM1 Antibody

SKU: orb1248571

Description

ICAM1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147), and other commercial sources. Recommended concentration: 1-3µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 cells. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SNCPDGQSTAKT
• Target: ICAM1
• Molecular Weight: Approx 100kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

orb1248571 (2 ug/ml) staining of HeLa (A) and Daudi (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248571 (2 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248571 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/membrane staining.

Quick Database Links

Gene Symbol

ICAM1

UniProt

P05362

RefSeq (Protein):NP_000192.2