CD50 Antibody / ICAM-3

SKU: orb639718

Description

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.

Research Area

Epigenetics

Images & Validation

• Tested Applications: Blocking, FACS, IF
• Dilution Range: Blocks binding of CD11a/LFA-1 to CD50/ICAM-3 (order BSA/azide-free format),Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD50 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CG106
• Immunogen: Stimulated human leukocytes were used as the immunogen for the CD50 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free CD50 antibody (clone CG106) as confirmation of integrity and purity.

Quick Database Links

UniProt

P32942