ICAM-1/CD54 (Extracellular) Antibody

• Catalog Number: orb1294365
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ICAM-1
• Target: ICAM-1 / CD54 (Extracellular)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human ICAM-1 / CD54
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.

ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ICAM-1 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 Anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 Anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.