Iba1, monoclonal Antibody

• Catalog Number: orb1294250
• Category: Antibodies
• Description: Mouse Monoclonal antibody to Iba1
• Target: Iba1, monoclonal
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:50-1:100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Iba1 monoclonal antibody orb1294250. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue.

Iba1 antibody at 1/500 dilution, Lysates/at 50 μg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294250 overnight at 4°C.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294250): 1-200. DAPI (blue) was used as the nuclear counter stain.