Hydralazine hydrochloride

SKU: orb1225370

Description

Hydralazine hydrochloride is a hydrochloride salt of hydralazine (Apresoline) that is a direct-acting smooth muscle relaxant.

Key Properties

• CAS Number: 304-20-1
• MW: 196.64
• Purity: >98% (HPLC)
• Formula: C₈H₉ClN₄
• SMILES: C1=CC=C2C(=C1)C=NN=C2NN.Cl
• Target: MAO
• Solubility: Water: 1 mg/mL (5.08 mM)

Bioactivity

• In Vivo:
  Animal model: Spinal cord injury mice. Dosage: 5 mg/kg. Administration: Intraperitoneal injection (i.p.). Result: Improved not only motor function and hypersensitivity but also spontaneous pain and emotion response in SCI mice. Inhibited both of BMDM recruitment and acrolein accumulation in the lesion epicenter of spinal cord in mice. Animal model: Spontaneously hypertensive rats (SHR). Dosage: 14 mg/kg. Administration: Supplemented in drinking water. Result: Reduced systolic blood pressure values and the number of adherent and migrating leukocytes in SHR. Reduce the number of adherent and migrating leukocytes in SHR. Decreased the levels of ICAM-1 mRNA.
• In Vitro:
  Cell Viability Assay Cell line: Human peritoneal mesothelial cell (HPMC). Concentration: 3 μg/mL, 6 μg/mL, 12 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL. Incubation time: 5 days. Result: Caused a dose-dependent inhibition of HPMC growth. Apoptosis Analysis Cell line: Jurkat, CEM-6, MOLT-4. Concentration: 40 μM, 80 μM, 200 μM, 400 μM, 600 μM. Incubation time: 16 h, 24 h, 48 h. Result: Observed significant apoptosis in Jurkat and MOLT-4 cells at concentrations of 200 μM. Induced a slight decrease in DNMT1 protein expression at 24 hr and nearly a complete loss after 48 hr. Observed proteolytic processing of the initiator caspases-8 and -9, as well as cleavage of the effector caspase-3.Resulted in generation of ROS and disruption of ΔΨm in Jurkat cells. Observed that overexpression of Bcl-2 and Bcl-xL proteins prevented cell death.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1-Hydrazinophthalazine

Quick Database Links

Gene Symbol

MAO