HUS1B Antibody

SKU: orb345458

Description

Hus1B antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-HUS1B antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein.
• Purity: This affinity-purified antibody is directed against human Hus1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.75 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-HUS1B antibody, Checkpoint protein HUS1B, hHUS1B, HUS 1B, HUS-1B, HUS1 Checkpoint Clamp Component B, HUS1B, HUS1 (S. Pombe) Checkpoint Homolog B, HUS1 Checkpoint Homolog B (S. Pombe), HUS1 Checkpoint Homolog B

Western blot using Biorbyt's affinity purified anti-Hus1B antibody shows detection of a 36kDa band [arrow] corresponding to Hus1B in a HeLa cell lysate (p/n orb348668). The staining pattern in 293 cells (p/n orb348669) is less clear, showing a predominant band at 39 kDa.

Quick Database Links

UniProt

Q8NHY5

RefSeq:31077215