HUPF1 Recombinant Rabbit Monoclonal Antibody

SKU: orb2563089

Description

HUPF1 Recombinant Rabbit Monoclonal Antibody

Research Area

DNA and RNA Research, RNA Processing

Images & Validation

• Tested Applications: FC, ICC, IP
• Dilution range: IP=1:10-50, Flow-Cyt=1ug/Test, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 8B15
• Immunogen: A synthesized peptide derived from human Regulator of nonsense transcripts 1 (1-53aa)
• Target: UPF1
• Molecular Weight: 124 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ATP dependent helicase RENT1; ATP-dependent helicase RENT1; delta helicase; HUPF 1; HUPF1; KIAA0221; Nonsense mRNA reducing factor 1; NORF 1; NORF1; pNORF 1; pNORF1; Regulator of nonsense transcripts 1; RENT 1; RENT1; RENT1_HUMAN; Smg 2; Smg 2 homolog nonsense mediated mRNA decay factor; UP Frameshift 1; Up frameshift mutation 1 homolog(S. cerevisiae); Up frameshift mutation 1 homolog; Up frameshift suppressor 1 homolog; Up-frameshift mutation 1 homolog(S. cerevisiae); Up-frameshift suppressor 1 homolog; UPF 1; UPF 1 regulator of nonsense transcripts homolog; UPF1.

4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Regulator of nonsense transcripts 1) monoclonal Antibody, unconjugated (orb2563089) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-Regulator of nonsense transcripts 1 antibody (orb2563089): 1 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

UPF1