Human/Mouse Myl9/12 (F6) rabbit mAb Antibody

SKU: orb1946351

Description

Myosin regulatory light chain (Myl) 9 is a regulatory subunit of the ATPase myosin protein. Myl9 regulates actin rearrangement to direct cellular migration, shape, and adhesion. Myl9 itself is regulated by post-translational modifications, including phosphorylation, acetylation and methylation. Phosphorylation of Myl9 at Thr18 and Ser19 promotes myosin ATPase activity and interaction with actin. Nα-acetylation of Myl9 has been shown to increase Ser19 phosphorylation and cytoplasmic activity, while Nα-methylation promotes DNA binding in the nucleus. Myl9, Myl12a, and Myl12b (Myl9/12) have been identified as functional ligands for CD69 in inflamed lungs, playing a major role in chronic inflammatory disorders such as chronic rhinosinusitis. Homozygous deletion in the MYL9 gene in humans has been identified as a putative molecular basis of the disease megacystis-microcolon-intestinal hypoperistalsis (MMIHS) syndrome, especially considering Myl9’s role in contracting smooth muscle cell.

Images & Validation

• Tested Applications: ELISA, FA, IHC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: AWBMyl9F6 (F-6)
• Immunogen: N-terminal peptide of Myl9
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1

Anti-human/mouse Myl9/12 antibody AWBMyl9F6 (Cat. #orb1946351) shows strong and specific tissue staining by immunohistochemistry.

Quick Database Links

UniProt

P24844