TSHR/LGR3 Mouse Monoclonal Antibody

SKU: orb2974494

Description

Anti-Human TSHR/LGR3 Antibody (B3J9) is a mouse monoclonal antibody detecting Thyroid-stimulating hormone receptor in FCM, Neutralization, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Images & Validation

• Tested Applications: FC, NeA, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B3J9
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3

Flow-cytometry using anti-human TSHR antibody. TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # orb2974494, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-TSHR monoclonal antibody at 1 µg/mL on various samples. Lane 1: Mouse ovary lysate.

Quick Database Links

UniProt

P16473