Human Stem Cell Factor Receptor (SCFR) ELISA Kit

SKU: orb775047

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor(SCFR).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CD117 ELISA Kit, KIT ELISA Kit, C-Kit ELISA Kit, PBT ELISA Kit, p145 c-Kit ELISA Kit, C-Kit Receptor ELISA Kit, Tyrosine-Protein Kinase ELISA Kit, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog ELISA Kit, Piebald Trait ELISA Kit

Standard curve for orb775047 ELISA kit.

Quick Database Links

UniProt

P10721