c-Kit (phospho Tyr721) rabbit pAb

• Catalog Number: orb764164
• Category: Antibodies
• Description: Rabbit polyclonal antibody to c-Kit (phospho-Tyr721)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human c-Kit around the phosphorylation site of Tyr721. AA range:688-737
• MW: 110
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-KIT antibody, anti-SCFR antibody, anti-Mast/s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-c-Kit (Y721) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-Kit (Phospho-Tyr721) Antibody.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using c-Kit (Phospho-Tyr721) Antibody. The lane on the right is blocked with the phospho peptide.