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Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) ELISA Kit

Catalog Number: orb776042

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776042
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha(IL2Ra).
Tested applicationsELISA
ReactivityHuman
Range15.63-1000 pg/mL
Assay Time3.5h
Concentration1000 pg/mL
Sensitivity6.7 pg/mL
Alternative namesCD25 ELISA Kit, IL2R-a ELISA Kit, IL2-RA ELISA Kit
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NoteFor research use only
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) ELISA Kit