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Catalog Number | orb776042 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha(IL2Ra). |
Tested applications | ELISA |
Reactivity | Human |
Range | 15.63-1000 pg/mL |
Assay Time | 3.5h |
Concentration | 1000 pg/mL |
Sensitivity | 6.7 pg/mL |
Alternative names | CD25 ELISA Kit, IL2R-a ELISA Kit, IL2-RA ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
Expiration Date | Please enquire. |
Human | |
93.75-6000 pg/mL | |
37.66 pg/mL |