Cart summary

You have no items in your shopping cart.

Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) ELISA Kit

Catalog Number: orb776042

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776042
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha(IL2Ra).
ReactivityHuman
Concentration1000 pg/mL
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity6.7 pg/mL
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Alternative namesCD25 ELISA Kit, IL2R-a ELISA Kit, IL2-RA ELISA Kit
Read more...
NoteFor research use only
Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) ELISA Kit