You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb2302948 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 93.75-6000 pg/mL |
Assay Time | 1.5h |
Concentration | 6000 pg/mL |
Sensitivity | 37.66 pg/mL |
Alternative names | IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; Read more... |
Note | For research use only |
Application notes | standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma |
Expiration Date | Please enquire. |