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Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep ELISA Kit

Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep ELISA Kit

Catalog Number: orb2302948

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2302948
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityHuman
Range93.75-6000 pg/mL
Assay Time1.5h
Concentration6000 pg/mL
Sensitivity37.66 pg/mL
Alternative namesIL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR;
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NoteFor research use only
Application notesstandard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma
Expiration DatePlease enquire.