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Human Siglec-3 Protein, Fc Tag
Catalog Number: orb1496138
| Catalog Number | orb1496138 |
|---|---|
| Category | Proteins |
| Description | Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cD encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia. |
| Reactivity | Human |
| Tag | C-Fc |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 53.1 kDa |
| Target | Siglec-3 |
| Protein Sequence | AAH28152.1 (R119G) |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Asp 18 - His 259 |
| Endotoxins | 0.2 EU per μg |
| NCBI | AAH28152.1, R119 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5 |
| Note | For research use only |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 64-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
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Mouse CD33 Protein, hFc Tag [orb1173692]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide.The apparent molecular mass of mCD33-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg Human CD33 protein [orb391407]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μgRecombinant Human CD33/Siglec-3 Protein, Fc & His Tag [orb1551311]
> 97% by SDS-PAGE.
Recombinant Human CD33/Siglec-3 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp18-His Tag259) of human CD33/Siglec-3 (Accession #NP_001763.3) fused with an Fc, 6��His Tag at the C-terminus.
100 μg, 50 μg
