Recombinant Human Siglec-3 Protein

SKU: orb2992990

Description

Recombinant Human Myeloid cell surface antigen CD33 is produced by our Mammalian expression system and the target gene encoding Asp18-His259 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human Siglec-3 (C-mFc) (Cat#orb2992990) at 2μg/ml (100 μl/well) can bind Anti-Human CD33 Antibody (Gemtuzumab ozogamicin)*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) is 7.679 ng/ml. (Regularly tested)
• Tag: C-mFc
• Molecular Weight: Predicted: 53.5 kDa. Observed: 65-90 kDa, reducing conditions
• Expression Region: Asp18-His259
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3

Quick Database Links

UniProt

AAH28152.1