ALB Antibody (HRP)

SKU: orb2652782

Description

ALB Antibody (HRP)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Human Serum Albumin Peroxidase antibody has been tested by ELISA and western blotting. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human Serum Albumin Antibody was produced by repeated immunizations with Human Serum Albumin.
• Target: ALB
• Purity: Human Serum Albumin antibody was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-human serum albumin antibody Peroxidase conjugation, HRP conjugated rabbit anti-HSA antibody, Peroxidase conjugated rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody HRP conjugation, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody

Western blot of Peroxidase conjugated Rabbit Anti-Human Serum Albumin secondary antibody. Lane 1: Human Serum Albumin (HSA). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase conjugated Rabbit Anti-Human Serum Albumin secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted\Observed size: 66 kDa, 61 kDa for HSA. Other band(s): None.

Quick Database Links

Gene Symbol

ALB

UniProt

P02768

RefSeq (Protein):NP_000468.1