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Human SCF Protein

Catalog Number: orb1496171

DispatchUsually dispatched within 5-10 working days
$ 380.00
Catalog Numberorb1496171
CategoryProteins
DescriptionStem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same R transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.
TargetSCF
ConjugationUnconjugated
TagNative
ReactivityHuman
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Purity95%
Protein SequenceGlu 26 - Ala 189
UniProt IDP21583
MW18.5 kDa
Application notesThis protein carries no "tag". The protein has a calculated MW of 18.5 kDa. The protein migrates as 30 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression SystemHEK293
Endotoxins0.01 EU per μg
Biological OriginHuman
Biological ActivityImmobilized Human SCF (26-189), premium grade (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD117, His, (Cat. No. CD7-H82E6) with a linear range of 0.005-0.3 μg/mL (QC tested).
Expression RegionGlu 26 - Ala 189
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
NoteFor research use only
Human SCF Protein

Immobilized Human SCF (26-189), premium grade (Cat. No. orb1496171) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD117, His, Avitag with a linear range of 0.005-0.3 μg/mL (QC tested).

  • Mouse SCF Protein, His Tag [orb257825]

    Unconjugated

    95%

    19.5 kDa

    50 μg, 20 μg, 1 mg
  • Human SCF Protein, His Tag [orb1743048]

    Unconjugated

    90%

    20.3 kDa

    1 mg, 100 μg
  • Human c-Kit ELISA Kit [orb219270]

    Human

    156 pg/ml - 10000 pg/ml

    80 pg/ml

    96 T
  • Human c-Kit ELISA Kit [orb219535]

    Human

    31.2 pg/ml - 2000 pg/ml

    2 pg/ml

    96 T
  • Human SCF protein (Active) [orb359026]

    > 97% as determined by SDS-PAGE and HPLC.

    18.5 kDa

    E.Coli

    10 μg, 100 μg, 500 μg