C-Kit/CD117 Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb499229
• Category: Antibodies
• Description: C-Kit/CD117 Rabbit Polyclonal Antibody (PE)
• Target: KIT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: PE
• Reactivity: Human, Mouse
• Predicted Reactivity: Equine
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human c-Kit/CD117 (451-550/976aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1μg /test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: C Kit; c-Kit; CD 117; CD117; CD117 antigen; KIT; K
• Note: For research use only

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb499229), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb499229), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.