Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit

SKU: orb781446

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2(Rock2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.113 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

KIAA0619 , p164 ROCK 2 , p164 ROCK-2 , Rho kinase 2 , Rho-associated , Rho-associated protein kinase 2 , ROCK 2 , Rock II , Rock2 , ROCK2, Rock2m , ROK alpha , ROKalpha

Quick Database Links

UniProt

O75116