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| Catalog Number | orb1736623 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Concentration | 20 ng/ml |
| UniProt ID | O75116 |
| Application notes | standard: 20 ng/ml. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.13 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | ROCK-II |
| Note | For research use only |
Human ROCK2 ELISA Kit [orb405812]
Human
0.625 ng/mL-40 ng/mL
7.8 pg/mL
48 T, 96 T, 10 x 96 T, 5 x 96 T, 24 T- Item 1 of 1
Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit [orb779326]
Human
0.16-10 ng/mL
0.059 ng/mL
48 T, 96 T - Item 1 of 1
Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit [orb781446]
Human
0.32-20 ng/mL
0.113 ng/mL
96 T, 48 T Human Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) ELISA Kit [orb1663746]
Human
48 T, 96 T
