Human Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

SKU: orb777284

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha(REG3a).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REG3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REG3a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.5 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HIP ELISA Kit, INGAP ELISA Kit, PAP-H ELISA Kit, PAP1 ELISA Kit, PBCGF ELISA Kit, REG-III ELISA Kit, REG3 ELISA Kit, Pancreatitis-Associated Protein ELISA Kit, Hepatointestinal pancreatic protein ELISA Kit, Human proislet peptide ELISA Kit, Pancreatitis-associated protein 1 ELISA Kit

Quick Database Links

UniProt

Q06141