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Mouse Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

Mouse Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

Catalog Number: orb2667536

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667536
CategoryAssays and Kits
DescriptionMouse Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit
Range0.32-20 ng/mL
Assay Time3.5h
UniProt IDO09037
Sensitivity0.11 ng/mL
Alternative namesHIP; INGAP; PAP-H; PAP1; PBCGF; REG-III; REG3; Pan
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG3a in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma and other biological fluids
Expiration DatePlease enquire.