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Human Phospholipase A1 (PLA1) ELISA Kit

Catalog Number: orb1947371

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1947371
CategoryAssays and Kits
DescriptionHuman Phospholipase A1 (PLA1) ELISA Kit
ReactivityHuman
Range31.25-2000pg/mL
Assay Time3.5H
TargetPLA1
UniProt IDQ53H76
Sensitivity18.75pg/mL
Alternative namesPLA1A, NMD, PSPLA1
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLA1. You can calculate the concentration of Human PLA1 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Human Phospholipase A1 (PLA1) ELISA Kit

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