Human PLA1(Phospholipase A1) Microsample ELISA Kit

SKU: orb2998981

Description

Human PLA1(Phospholipase A1) Microsample ELISA Kit

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research, Protein Biochemistry

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PLA1A; ps-PLA1; NMD; Phosphatidylserine-Specific Phospholipase A1-Alpha

Quick Database Links

UniProt

Q53H76