Human Peptidase Inhibitor 3, Skin Derived (PI3) ELISA Kit

SKU: orb777026

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidase Inhibitor 3, Skin Derived(PI3).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, urine, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.127 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

SKALP ELISA Kit, ESI ELISA Kit, WAP3 ELISA Kit, WFDC14 ELISA Kit, Elafin ELISA Kit, Skin-Derived Antileukoproteinase ELISA Kit, Cementoin ELISA Kit, Elastase-specific inhibitor ELISA Kit, WAP four-disulfide core domain protein 14 ELISA Kit

Quick Database Links

UniProt

P19957