Human Elafin (PI3) ELISA Kit

SKU: orb1291361

Description

Human PI3 (Elafin) ELISA kit

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.8 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Cementoin, ELAF_HUMAN, Elafin, Elafin/Skalp, Elastase Specific Inhibitor, Elastase-specific inhibitor, ES 1, ESI, Peptidase inhibitor 3, Peptidase inhibitor 3, skin derived, PI 3, PI-3, PI3, Pre elafin, Protease inhibitor 3 skin derived, Protease inhibitor 3, skin derived (SKALP), Protease inhibitor WAP3, SKALP, Skin derived Anti leukoproteinase, Skin-derived antileukoproteinase, Trappin 2, WAP four disulfide core domain 14, WAP four disulfide core domain protein 14, WAP four-disulfide core domain protein 14, WAP3, WFDC14

Quick Database Links

UniProt

P19957