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Catalog Number | orb653660 |
---|---|
Category | Proteins |
Description | Human NKG2D, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1). |
Target | NKG2D |
Conjugation | Unconjugated |
Tag | N-mFc(mIgG2a) |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_031386.2 (T72A) |
UniProt ID | P26718 |
MW | 43.5 kDa |
Application notes | This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Expression Region | Ile 73 - Val 216 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | T72, NP_031386.2 |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-NKG2D is approximately 50-65 kDa due to glycosylation. | |
Mammalian |
98.00% | |
36.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
41.83 kDa (predicted); 46.51 kDa (reducing conditions) |