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Catalog Number | orb429438 |
---|---|
Category | Proteins |
Description | Recombinant of human MCP1 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | CCL2 was lyophilized from a concentrated (1mg/ml) sterile solution without any additives. |
Purity | Greater than 98.0% as determined by RP-HPLC and SDS-PAGE. |
Protein Sequence | QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT |
Application notes | Chemokines |
Source | Escherichia Coli |
Biological Activity | The specific activity as determined by the ability of MCP-1 to chemoattract human THP-1 cells is detectable starting at 1ng/ml. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Small inducible cytokine A2, CCL2, Monocyte chemot Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
> 96% as determined by SDS-PAGE and HPLC. | |
8.7 kDa | |
E.Coli |
> 96% as determined by SDS-PAGE and HPLC. | |
8.6 kDa | |
E.Coli |
> 96 % by SDS-PAGE and HPLC analyses. | |
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. | |
Escherichia coli |
The human full length CCR2 protein has a MW of 41.9 kDa | |
Mammalian |