Recombinant Human KGF Protein

SKU: orb3002467

Description

Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: ELISA: Immobilized Human KGF(Cat. No.:orb3002467) at 5 μg/mL (100 μL/well) can bind Human FGFR2 beta IIIb (C-Fc)(Cat. No.:orb2993044). The EC50 of Human FGFR2 beta IIIb (C-Fc)(Cat. No.:orb2993044) is 83 ng/mL.（Regularly tested）. Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494) , R-spondin 3 (Cat#orb2993001) , KGF(Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753). DAPI (blue), Ki67(red) and ER(green).
• Tag: Tag free
• Expression Region: Cys32-Thr194
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 18.9 KDa. Observed: 17 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF

Quick Database Links

UniProt

P21781