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Human FGF-10
SKU: orb3002478
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured by its ability to induce FGFRIIIb receptor activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 3.82ng/ml (Regularly tested). Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#orb3002493) , R-spondin 1 (Cat#orb3002497) ,FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483). The organoids showed good morphology. Human breast cancer organoids were cultured with Wnt3a(Cat#orb3002512),Noggin (Cat#orb3002494),R-spondin 3 (Cat#orb2993001),KGF(Cat#orb3002467), FGF-10(Cat#orb3002478),EGF (Cat#orb3002493)and NRG1-beta 1(Cat#753). DAPI (blue), Ki67(red) and ER(green). The activity of Recombinant Human FGF-10 Protein(Cat#orb3002478) was compared to Brand R. |
| Tag | Tag free |
| Molecular Weight | Predicted: 19.5 KDa. Observed: 19-22 KDa, reducing conditions |
| Expression Region | Gln38-Ser208 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 10mM Tris, 5% Sucrose, 4% Mannitol, 0.02% Tween80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2
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Human FGF-10 (orb3002478)
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