Human Interleukin 10 (IL10) ELISA Kit

SKU: orb775007

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10(IL10).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL10 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CSIF ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Cytokine Synthesis Inhibitory Factor ELISA Kit

Quick Database Links

UniProt

P22301

References

R Paduch, M Klatka, P Pieniądz, I Wertel, A Pawłowska, J Klatka Reciprocal Interactions of Human Monocytes and Cancer Cells in Co-Cultures In Vitro Current Issues in Molecular Biology, (2024)

PubMed:

Applications

ELISA

Reactivity

Human