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Human Interleukin 10 (IL10) ELISA Kit

Catalog Number: orb775007

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775007
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10(IL10).
ReactivityHuman
Concentration500 pg/mL
UniProt IDP22301
Tested applicationsELISA
Application notesstandard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL10 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range7.82-500 pg/mL
Sensitivity3 pg/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesCSIF ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, C
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NoteFor research use only

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R Paduch, M Klatka, P Pieniądz, I Wertel, A Pawłowska, J Klatka Reciprocal Interactions of Human Monocytes and Cancer Cells in Co-Cultures In Vitro Current Issues in Molecular Biology, (2024)

Applications

ELISA

Reactivity

Human
Human Interleukin 10 (IL10) ELISA Kit

  • Human IL-10/Interleukin-10 ELISA Kit [orb50055]

    Human

    7.8 pg/ml - 500 pg/ml

    < 0.5 pg/ml

    96 T
  • Human IL-10 ELISA Kit [orb561774]

    Human

    7.813-500pg/ml

    4.688pg/ml

    96 T, 48 T
  • Human IL10 ELISA Kit [orb405959]

    Human

    31.25 pg/ml - 2000 pg/ml

    7.8 pg/ml

    5 x 96 T, 48 T, 96 T, 10 x 96 T, 24 T
  • Human Interleukin 10 Receptor Beta (IL10Rb) ELISA Kit [orb778932]

    Human

    78.13-5000 pg/mL

    30 pg/mL

    96 T, 48 T
  • Human Interleukin 10 Receptor Alpha (IL10Ra) ELISA Kit [orb777848]

    Human

    31.25-2000 pg/mL

    11.6 pg/mL

    96 T, 48 T