Human Interleukin 10 Receptor Alpha (IL10Ra) ELISA Kit

SKU: orb777848

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Alpha(IL10Ra).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL10Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL10Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL10Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL10Ra in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 11.6 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CDW210A ELISA Kit, CD210A ELISA Kit, IL10R-A ELISA Kit, IL10-RA ELISA Kit, CD210-A ELISA Kit, HIL-10R ELISA Kit, IL-10R1 ELISA Kit, IL10R ELISA Kit, Cluster Of Differentiation W210A ELISA Kit

Quick Database Links

UniProt

Q13651