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Catalog Number | orb611009 |
---|---|
Category | Proteins |
Description | Recombinant Human IL22 protein |
Form/Appearance | Sterile filtered colorless solution. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | ADPAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACIH HHHHH |
Source | Sf9, Baculovirus cells |
Biological Activity | The ED50 as determined by its ability to induce IL-10 secretion using COLO 205 human colorectal adenocarcinoma cell is ≤ 1.2 ng/ml. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | IL22 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 10% glycerol. |
Alternative names | IL-TIF, TIFa, IL-10-related T-cell-derived-inducib Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
16.9 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.9 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.9 kDa after removal of the signal peptide. The apparent molecular mass of IL22-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
> 90% as determined by SDS-PAGE | |
17.8 kDa |