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Catalog Number | orb257626 |
---|---|
Category | Proteins |
Description | Human IL-4 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1). |
Target | IL-4 R alpha |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_000409.1 |
UniProt ID | P24394 |
MW | 24.6 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-4 R alpha, His Tag (Cat. No. ) at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, ,His Tag (Cat. No. IL4-H82E0) with a linear range of 2-16 ng/mL (QC tested). |
Expression Region | Met 26 - His 232 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000409.1 |
SDS-PAGE analysis of Human IL-4 R alpha protein
Immobilized Human IL-4 R alpha, His Tag (Cat. No. orb257626) at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag, His Tag with a linear range of 2-16 ng/mL (QC tested).
Unconjugated | |
95% | |
50.2 kDa & 63.1 kDa |